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dc.date.accessioned2022-04-06T13:55:59Z
dc.date.available2015-02-17T15:38:03Zen
dc.date.available2022-04-06T13:55:59Z
dc.date.issued1975-09-01en
dc.identifier.issn0006-2952
dc.identifier.issn0300-9629
dc.identifier.issn0148-4834
dc.identifier.issn0006-291X
dc.identifier.issn0006-3002
dc.identifier.pmid10
dc.identifier.pmid1186
dc.identifier.pmid1477
dc.identifier.pmid7525
dc.identifier.pmid5
dc.identifier.pmid63
dc.identifier.urihttp://hdl.handle.net/2384/344536
dc.description.abstract1. Crude extracts and partially purified enzyme preparations from potato tubers catalyse, at pH 5-7, the conversion of linoleic acid hydroperoxides to a range of oxygenated fatty acid derivatives. 2. 9-D- and 13-L-hydroperoxide isomers are converted at similar rates to equivalent (isomeric) products. 3. The major products from the 13-hydroperoxide isomer were identified as the corresponding monohydroxydienoic acid derivative, threo-11-hydroxy-trans12,13-epoxy-octadec-cis9-enoic acid and 9,12,13-trihydroxy-octadec-trans10-enoic acid. The corresponding products from the 9-hydroperoxide were the monohydroxydienoic acid, 9,10-epoxy-11-hydroxy-octadec-12-enoic acid and 9,10,13-trihydroxy-octadec-11-enoic acid. 4. No separation of activities forming the different products was achieved by partial purification of enzyme extracts. 5. Product formation was unaffected by EDTA, CN-, sulphydryl reagents or glutathione but was reduced by boiling the extracts. 6. This system is compared with the 9-hydroperoxide-specific enzymic formation of divinyl ether derivatives by potato extracts.
dc.description.sponsorshipljh/'oihpihpihj#pih#0-en_US
dc.language.isoenen_US
dc.rightsArchived with thanks to Biochemical pharmacologyen
dc.subject.meshAnimalsen
dc.subject.meshChromatography, Thin Layeren
dc.subject.meshDigitoxigeninen
dc.subject.meshDigitoxinen
dc.subject.meshHydroxylationen
dc.subject.meshIn Vitro Techniquesen
dc.subject.meshMaleen
dc.subject.meshMicrosomes, Liveren
dc.subject.meshNADPen
dc.subject.meshRatsen
dc.subject.meshTime Factorsen
dc.subject.meshAdrenalectomyen
dc.subject.meshAnimalsen
dc.subject.meshBiological Transport, Activeen
dc.subject.meshGlucoseen
dc.subject.meshLiver Glycogenen
dc.subject.meshRatsen
dc.subject.meshTrypanosomaen
dc.subject.meshBook Selectionen
dc.subject.meshEducation, Nursingen
dc.subject.meshHistory, 19th Centuryen
dc.subject.meshHistory, 20th Centuryen
dc.subject.meshIllinoisen
dc.subject.meshSocieties, Medicalen
dc.subject.meshAnimalsen
dc.subject.meshCnidariaen
dc.subject.meshComputersen
dc.subject.meshHemerythrinen
dc.subject.meshMetalloproteinsen
dc.subject.meshModels, Molecularen
dc.subject.meshMuscle Proteinsen
dc.subject.meshProtein Conformationen
dc.subject.meshSpecies Specificityen
dc.subject.meshFatty Acids, Unsaturateden
dc.subject.meshHydrogen-Ion Concentrationen
dc.subject.meshLinoleic Acidsen
dc.subject.meshMagnetic Resonance Spectroscopyen
dc.subject.meshPeroxidasesen
dc.subject.meshPlantsen
dc.subject.meshStructure-Activity Relationshipen
dc.titleDigitoxin metabolism by rat liver microsomes@1.en_US
dc.identifier.journalBiochemical pharmacology
refterms.dateFOA2022-10-25T12:51:45Z
html.description.abstract1. Crude extracts and partially purified enzyme preparations from potato tubers catalyse, at pH 5-7, the conversion of linoleic acid hydroperoxides to a range of oxygenated fatty acid derivatives. 2. 9-D- and 13-L-hydroperoxide isomers are converted at similar rates to equivalent (isomeric) products. 3. The major products from the 13-hydroperoxide isomer were identified as the corresponding monohydroxydienoic acid derivative, threo-11-hydroxy-trans12,13-epoxy-octadec-cis9-enoic acid and 9,12,13-trihydroxy-octadec-trans10-enoic acid. The corresponding products from the 9-hydroperoxide were the monohydroxydienoic acid, 9,10-epoxy-11-hydroxy-octadec-12-enoic acid and 9,10,13-trihydroxy-octadec-11-enoic acid. 4. No separation of activities forming the different products was achieved by partial purification of enzyme extracts. 5. Product formation was unaffected by EDTA, CN-, sulphydryl reagents or glutathione but was reduced by boiling the extracts. 6. This system is compared with the 9-hydroperoxide-specific enzymic formation of divinyl ether derivatives by potato extracts.


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