Digitoxin metabolism by rat liver microsomes@1.
dc.date.accessioned | 2022-04-06T13:55:59Z | |
dc.date.available | 2015-02-17T15:38:03Z | en |
dc.date.available | 2022-04-06T13:55:59Z | |
dc.date.issued | 1975-09-01 | en |
dc.identifier.issn | 0006-2952 | |
dc.identifier.issn | 0300-9629 | |
dc.identifier.issn | 0148-4834 | |
dc.identifier.issn | 0006-291X | |
dc.identifier.issn | 0006-3002 | |
dc.identifier.pmid | 10 | |
dc.identifier.pmid | 1186 | |
dc.identifier.pmid | 1477 | |
dc.identifier.pmid | 7525 | |
dc.identifier.pmid | 5 | |
dc.identifier.pmid | 63 | |
dc.identifier.uri | http://hdl.handle.net/2384/344536 | |
dc.description.abstract | 1. Crude extracts and partially purified enzyme preparations from potato tubers catalyse, at pH 5-7, the conversion of linoleic acid hydroperoxides to a range of oxygenated fatty acid derivatives. 2. 9-D- and 13-L-hydroperoxide isomers are converted at similar rates to equivalent (isomeric) products. 3. The major products from the 13-hydroperoxide isomer were identified as the corresponding monohydroxydienoic acid derivative, threo-11-hydroxy-trans12,13-epoxy-octadec-cis9-enoic acid and 9,12,13-trihydroxy-octadec-trans10-enoic acid. The corresponding products from the 9-hydroperoxide were the monohydroxydienoic acid, 9,10-epoxy-11-hydroxy-octadec-12-enoic acid and 9,10,13-trihydroxy-octadec-11-enoic acid. 4. No separation of activities forming the different products was achieved by partial purification of enzyme extracts. 5. Product formation was unaffected by EDTA, CN-, sulphydryl reagents or glutathione but was reduced by boiling the extracts. 6. This system is compared with the 9-hydroperoxide-specific enzymic formation of divinyl ether derivatives by potato extracts. | |
dc.description.sponsorship | ljh/'oihpihpihj#pih#0- | en_US |
dc.language.iso | en | en_US |
dc.rights | Archived with thanks to Biochemical pharmacology | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Chromatography, Thin Layer | en |
dc.subject.mesh | Digitoxigenin | en |
dc.subject.mesh | Digitoxin | en |
dc.subject.mesh | Hydroxylation | en |
dc.subject.mesh | In Vitro Techniques | en |
dc.subject.mesh | Male | en |
dc.subject.mesh | Microsomes, Liver | en |
dc.subject.mesh | NADP | en |
dc.subject.mesh | Rats | en |
dc.subject.mesh | Time Factors | en |
dc.subject.mesh | Adrenalectomy | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Biological Transport, Active | en |
dc.subject.mesh | Glucose | en |
dc.subject.mesh | Liver Glycogen | en |
dc.subject.mesh | Rats | en |
dc.subject.mesh | Trypanosoma | en |
dc.subject.mesh | Book Selection | en |
dc.subject.mesh | Education, Nursing | en |
dc.subject.mesh | History, 19th Century | en |
dc.subject.mesh | History, 20th Century | en |
dc.subject.mesh | Illinois | en |
dc.subject.mesh | Societies, Medical | en |
dc.subject.mesh | Animals | en |
dc.subject.mesh | Cnidaria | en |
dc.subject.mesh | Computers | en |
dc.subject.mesh | Hemerythrin | en |
dc.subject.mesh | Metalloproteins | en |
dc.subject.mesh | Models, Molecular | en |
dc.subject.mesh | Muscle Proteins | en |
dc.subject.mesh | Protein Conformation | en |
dc.subject.mesh | Species Specificity | en |
dc.subject.mesh | Fatty Acids, Unsaturated | en |
dc.subject.mesh | Hydrogen-Ion Concentration | en |
dc.subject.mesh | Linoleic Acids | en |
dc.subject.mesh | Magnetic Resonance Spectroscopy | en |
dc.subject.mesh | Peroxidases | en |
dc.subject.mesh | Plants | en |
dc.subject.mesh | Structure-Activity Relationship | en |
dc.title | Digitoxin metabolism by rat liver microsomes@1. | en_US |
dc.identifier.journal | Biochemical pharmacology | |
refterms.dateFOA | 2022-10-25T12:51:45Z | |
html.description.abstract | 1. Crude extracts and partially purified enzyme preparations from potato tubers catalyse, at pH 5-7, the conversion of linoleic acid hydroperoxides to a range of oxygenated fatty acid derivatives. 2. 9-D- and 13-L-hydroperoxide isomers are converted at similar rates to equivalent (isomeric) products. 3. The major products from the 13-hydroperoxide isomer were identified as the corresponding monohydroxydienoic acid derivative, threo-11-hydroxy-trans12,13-epoxy-octadec-cis9-enoic acid and 9,12,13-trihydroxy-octadec-trans10-enoic acid. The corresponding products from the 9-hydroperoxide were the monohydroxydienoic acid, 9,10-epoxy-11-hydroxy-octadec-12-enoic acid and 9,10,13-trihydroxy-octadec-11-enoic acid. 4. No separation of activities forming the different products was achieved by partial purification of enzyme extracts. 5. Product formation was unaffected by EDTA, CN-, sulphydryl reagents or glutathione but was reduced by boiling the extracts. 6. This system is compared with the 9-hydroperoxide-specific enzymic formation of divinyl ether derivatives by potato extracts. |