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dc.contributor.authorSchmoldt, Aen
dc.contributor.authorBenthe, H Fen
dc.contributor.authorHaberland, Gen
dc.contributor.authorSanchez, Gen
dc.contributor.authorAlderete, J Fen
dc.contributor.authorBridgewater, Sen
dc.contributor.authorCamp, H Men
dc.contributor.authorHendrickson, W Aen
dc.contributor.authorWard, K Ben
dc.contributor.authorGalliard, Ten
dc.contributor.authorPhillips, D Ren
dc.contributor.authorMatthew, J Aen
dc.contributor.authorBundy, CLen
dc.date.accessioned2015-02-17T15:38:03Zen
dc.date.available2015-02-17T15:38:03Zen
dc.date.issued1975-09-01en
dc.identifier.citationDigitoxin metabolism by rat liver microsomes. 1975, 24, 52, 15, 150, 66, 409 (17) (4) (1) (1) (4) (2):1639-41:623-6:3-5:49-54:1349-56:157-71 Biochem. Pharmacol. Comp Biochem Physiol A Comp Physiol J Nurs Educ IMJ Ill Med J Biochem. Biophys. Res. Commun. Biochim. Biophys. Actaen
dc.identifier.issn0006-2952en
dc.identifier.issn0300-9629en
dc.identifier.issn0148-4834en
dc.identifier.issn0006-291Xen
dc.identifier.issn0006-3002en
dc.identifier.pmid10en
dc.identifier.pmid1186en
dc.identifier.pmid1477en
dc.identifier.pmid7525en
dc.identifier.pmid5en
dc.identifier.pmid63en
dc.identifier.urihttp://hdl.handle.net/2384/344536en
dc.description.abstract1. Crude extracts and partially purified enzyme preparations from potato tubers catalyse, at pH 5-7, the conversion of linoleic acid hydroperoxides to a range of oxygenated fatty acid derivatives. 2. 9-D- and 13-L-hydroperoxide isomers are converted at similar rates to equivalent (isomeric) products. 3. The major products from the 13-hydroperoxide isomer were identified as the corresponding monohydroxydienoic acid derivative, threo-11-hydroxy-trans12,13-epoxy-octadec-cis9-enoic acid and 9,12,13-trihydroxy-octadec-trans10-enoic acid. The corresponding products from the 9-hydroperoxide were the monohydroxydienoic acid, 9,10-epoxy-11-hydroxy-octadec-12-enoic acid and 9,10,13-trihydroxy-octadec-11-enoic acid. 4. No separation of activities forming the different products was achieved by partial purification of enzyme extracts. 5. Product formation was unaffected by EDTA, CN-, sulphydryl reagents or glutathione but was reduced by boiling the extracts. 6. This system is compared with the 9-hydroperoxide-specific enzymic formation of divinyl ether derivatives by potato extracts.
dc.description.sponsorshipljh/'oihpihpihj#pih#0-en
dc.language.isoenen
dc.rightsArchived with thanks to Biochemical pharmacologyen
dc.subject.meshAnimalsen
dc.subject.meshChromatography, Thin Layeren
dc.subject.meshDigitoxigeninen
dc.subject.meshDigitoxinen
dc.subject.meshHydroxylationen
dc.subject.meshIn Vitro Techniquesen
dc.subject.meshMaleen
dc.subject.meshMicrosomes, Liveren
dc.subject.meshNADPen
dc.subject.meshRatsen
dc.subject.meshTime Factorsen
dc.subject.meshAdrenalectomyen
dc.subject.meshAnimalsen
dc.subject.meshBiological Transport, Activeen
dc.subject.meshGlucoseen
dc.subject.meshLiver Glycogenen
dc.subject.meshRatsen
dc.subject.meshTrypanosomaen
dc.subject.meshBook Selectionen
dc.subject.meshEducation, Nursingen
dc.subject.meshHistory, 19th Centuryen
dc.subject.meshHistory, 20th Centuryen
dc.subject.meshIllinoisen
dc.subject.meshSocieties, Medicalen
dc.subject.meshAnimalsen
dc.subject.meshCnidariaen
dc.subject.meshComputersen
dc.subject.meshHemerythrinen
dc.subject.meshMetalloproteinsen
dc.subject.meshModels, Molecularen
dc.subject.meshMuscle Proteinsen
dc.subject.meshProtein Conformationen
dc.subject.meshSpecies Specificityen
dc.subject.meshFatty Acids, Unsaturateden
dc.subject.meshHydrogen-Ion Concentrationen
dc.subject.meshLinoleic Acidsen
dc.subject.meshMagnetic Resonance Spectroscopyen
dc.subject.meshPeroxidasesen
dc.subject.meshPlantsen
dc.subject.meshStructure-Activity Relationshipen
dc.titleDigitoxin metabolism by rat liver microsomes.en
dc.identifier.journalBiochemical pharmacologyen
html.description.abstract1. Crude extracts and partially purified enzyme preparations from potato tubers catalyse, at pH 5-7, the conversion of linoleic acid hydroperoxides to a range of oxygenated fatty acid derivatives. 2. 9-D- and 13-L-hydroperoxide isomers are converted at similar rates to equivalent (isomeric) products. 3. The major products from the 13-hydroperoxide isomer were identified as the corresponding monohydroxydienoic acid derivative, threo-11-hydroxy-trans12,13-epoxy-octadec-cis9-enoic acid and 9,12,13-trihydroxy-octadec-trans10-enoic acid. The corresponding products from the 9-hydroperoxide were the monohydroxydienoic acid, 9,10-epoxy-11-hydroxy-octadec-12-enoic acid and 9,10,13-trihydroxy-octadec-11-enoic acid. 4. No separation of activities forming the different products was achieved by partial purification of enzyme extracts. 5. Product formation was unaffected by EDTA, CN-, sulphydryl reagents or glutathione but was reduced by boiling the extracts. 6. This system is compared with the 9-hydroperoxide-specific enzymic formation of divinyl ether derivatives by potato extracts.


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