Now showing items 1-20 of 296

    • Waternoten Trapa natans L. van Maasvlakte 2

      Langeveld, Bram W.
      In dit artikel worden vondsten vermeld van een drijvende plant: de waternoot Trapa natans L. Komt in Nederland voor maar is inmiddels zeldzaam
    • Carbon-diversity hotspots and their owners in Brazilian southeastern Savanna, Atlantic Forest and Semi-Arid Woodland domains

      Silveira, Eduarda Martiniano de Oliveira; Terra, Marcela de Castro Nunes Santos; ter Steege, Hans; Maeda, Eduardo Eiji; Acerbi Júnior, Fausto Weimar; Scolforo, Jose Roberto Soares (2019-11-15)
    • Uncovering bacterial and functional diversity in macroinvertebrate mitochondrial-metagenomic datasets by differential centrifugation

      Macher, Jan Niklas; Speksnijder, Arjen; Choo, Le Qin; van der Hoorn, Berry; Renema, Willem (2019-12-01)
      PCR-free techniques such as meta-mitogenomics (MMG) can recover taxonomic composition of macroinvertebrate communities, but suffer from low efficiency, as >90% of sequencing data is mostly uninformative due to the great abundance of nuclear DNA that cannot be identified with current reference databases. Current MMG studies do not routinely check data for information on macroinvertebrate-associated bacteria and gene functions. However, this could greatly increase the efficiency of MMG studies by revealing yet overlooked diversity within ecosystems and making currently unused data available for ecological studies. By analysing six ‘mock’ communities, each containing three macroinvertebrate taxa, we tested whether this additional data on bacterial taxa and functional potential of communities can be extracted from MMG datasets. Further, we tested whether differential centrifugation, which is known to greatly increase efficiency of macroinvertebrate MMG studies by enriching for mitochondria, impacts on the inferred bacterial community composition. our results show that macroinvertebrate MMG datasets contain a high number of mostly endosymbiont bacterial taxa and associated gene functions. Centrifugation reduced both the absolute and relative abundance of highly abundant Gammaproteobacteria, thereby facilitating detection of rare taxa and functions. When analysing both taxa and gene functions, the number of features obtained from the MMG dataset increased 31-fold (‘enriched’) respectively 234-fold (‘not enriched’). We conclude that analysing MMG datasets for bacteria and gene functions greatly increases the amount of information available and facilitates the use of shotgun metagenomic techniques for future studies on biodiversity.
    • Key to the Malesian species of Mallotus (Euphorbiaceae)

      van Welzen, P.C.; Kulju, K.K.M.; Sierra, S.E.C.; Slik, J.W.F. (2010-12-09)
      An identification key to all Malesian species of Mallotus was still lacking. So far, only keys per section of Mallotus or keys per island were generated. This is the first attempt to create a key for all species of Mallotus in Malesia.
    • Zoogdieren in een veranderend landschap. NL in Beeld legt omgeving vast in foto’s

      van der Straaten, Jan
      Het maken van foto’s blijkt de beste manier om de kenmerken en de veranderingen van het landschap vast te leggen. Ook hiermee samenhangende veranderingen in populaties van zoogdieren kunnen zo worden gevolgd.
    • Development of a new continental-scale index for freshwater assessment based on dragonfly assemblages

      Vorster, Carlien; Samways, Michael J.; Simaika, John P.; Kipping, Jens; Clausnitzer, Viola; Suhling, Frank; Dijkstra, K. D.B. (2020-02-01)
    • The Charles Sturt University Remote Telescope Project

      Mckinnon, David (Our Solar Siblings, 2018-10-18)
    • Effect of nanofluid on CO2-wettability reversal of sandstone formation; implications for CO2 geo-storage

      Sahito, Muhammad Faraz; Jha, Nilesh Kumar; Arain, Zain-Ul-Abedin; Memon, Shoaib; Keshavarz, Alireza; Iglauer, Stefan; Saeedi, Ali; Sarmadivaleh, Mohammad (2020-02-01)
      HypothesisNanofluid treatment is a promising technique which can be used for wettability reversal of CO2-brine-mineral systems towards a further favourable less CO2-wet state in the existence of organic acids. However, literature requires more information and study with respect to organic acids and nanoparticles’ effect at reservoir (high pressure and high temperature) conditions. ExperimentsTherefore, we have measured in this study that what influence small amounts of organic acids exposed to quartz for aging time of (7 days and 1 year) have on their wettability and how this impact can be reduced by using different concentrations of nanoparticles at reservoir conditions. Precisely, we have tested lignoceric acid (C24), stearic acid (C18), lauric acid (C12) and hexanoic acid (C6) at 10−2 Molarity, as well as, we have also used different concentrations (0.75 wt%, 0.25 wt%, 0.1 wt%, 0.05 wt%) of silica nanoparticles at realistic storage conditions. FindingsThe quartz surface turned significantly hydrophobic when exposed to organic acids for longer aging time of 1 year, and significantly hydrophilic after nanofluid treatment at optimum concentration of 0.1 wt%. It was observed that most nanoparticles were mechanistically irreversibly adsorbed on the surface of quartz sample. This wettability shift thus may increase CO2 storage capacities and containment security.
    • Effect of nanofluid on CO<inf>2</inf>-wettability reversal of sandstone formation; implications for CO<inf>2</inf> geo-storage

      Ali, Muhammad; Sahito, Muhammad Faraz; Jha, Nilesh Kumar; Arain, Zain Ul Abedin; Memon, Shoaib; Keshavarz, Alireza; Iglauer, Stefan; Saeedi, Ali; Sarmadivaleh, Mohammad (2020-02-01)
    • A hybrid multi-criteria decision making method for site selection of subsurface dams in semi-arid region of Iran

      Amal, Dortaj Author, Two (2020-04-30)
      Water shortage is one of the most critical problems all around the world, especially in arid and semi-arid regions. Using groundwater can be an efficient solution in such lands. Although significant care and appropriate water resource management strategies are required to be implemented. Nowadays, subsurface dams (SSD) are widely used worldwide since they are proved to have advantages over surface dams in some cases. Selection of the suitable site for SSD construction is critical. This selection is made based on advantages and disadvantages of each location. Multi-criteria decision-making methods (MCDM) is an efficient method for optimum site selection. In this study, 10 regions were chosen as alternatives for construction of subsurface dams in Isfahan province of Iran. These alternatives were then ranked using ELECTRE I, II and modified ELECTRE III based on geological, hydrological, climatological and socio-economical criteria. Results from different ELECTRE methods are combined by applying the grade average, and Borda and Copeland ranking strategies. Application of an advanced MCDM method reduced uncertainties in subsurface dams (SSD) site selection. Alternative 5 (Hoseinabad) was introduced as the best location for subsurface dam construction. This methodology can be used as a basis for more detailed field investigations.
    • Neurophysiological and Psychological Predictors of Social Functioning in Patients with Schizophrenia and Bipolar Disorder.

      Kim, Yourim; Kwon, Aeran; Min, Dongil; Kim, Sungkean; Jin, Min Jin; Lee, Seung-Hwan (2019-10-07)
    • Identifying labelling and marketing advantages of nutrients in minced beef meat: A case study

      Oostindjer, Marije; Hovland, Ellen Margrethe; Okholm, Bolette; Saarem, Kristin; Bjerke, Frøydis; Ruud, Lene; Grabež, Vladana; Haug, Anna; Brian James (2020-01-01)
    • Development status of Terumo implantable left ventricular assist system.

      Nojiri, C; Kijima, T; Maekawa, J; Horiuchi, K; Kido, T; Sugiyama, T; Mori, T; Sugiura, N; Asada, T; Umemura, W; et al. (2001-05-01)
      We have been developing an implantable left ventricular assist system (T‐ILVAS) featuring a magnetically suspended centrifugal pump (MSCP) since 1995. In vitro and in vivo studies using a prototype MSCP composed of a polycarbonate housing and impeller (196 ml) have demonstrated long‐term durability and excellent blood compatibility for up to 864 days, and excellent stability of the magnetic bearing of the MSCP. These preliminary results strongly suggested that the magnetic bearing of the MSCP is reliable and is a most feasible mechanism for a long‐term circulatory assist device. We have recently devised a clinical version pump made of titanium (180 ml) with a new position sensor mechanism and a wearable controller with batteries. Cadaver fit study confirmed that the Type IV pump could be implanted in a small patient with a body surface area as small as 1.3. The in vitro performance tests of the Type IV pump demonstrated excellent hydrodynamic performances with an acceptable hemolysis rate. New position sensors for the titanium housing showed more uniform sensor outputs of a magnetic bearing than in the prototype polycarbonate pump. The Type IV pump then was evaluated in vivo in 6 sheep at the Oxford Heart Centre. Four sheep were electively sacrificed at 3 months and were allowed to survive for more than 6 months for long‐term evaluation. In this particular series of experiments, no anticoagulant/antiplatelet regimen was utilized except for a bolus dose of heparin during surgery. There was a left ventricular mural thrombi around the inflow cannula in 1 sheep. Otherwise, there was no mechanical failure nor sign of thromboembolism throughout the study.
    • A comparison of automated perfusion- and manual diffusion-based human regulatory T cell expansion using a soluble activator complex

      Nankervis, B.; Vang, B.; Frank, N.; Coeshott, C. (2019-05-31)
      Background & Aim Human regulatory T cells (hTregs) play a central role in the maintenance of self-tolerance and suppression of autoimmune responses. The absence or reduced frequency of hTregs can limit the control of immune inflammatory responses, autoimmunity and the success of transplant engraftment. Clinical studies indicate that the use of hTregs as immunotherapeutics would require billions of cells per dose; e.g., Phase I studies by Mathew et al (2018) suggest that hTreg doses in the range of 0.5 to 5.0 × 109 cells are needed to facilitate kidney transplantation and clinical studies by Bluestone et al (2015) indicate that polyclonal hTreg doses in the range of 5 × 106 to 2.6 × 109 cells are well tolerated in type 1 diabetes patients. The Quantum Cell Expansion System is a hollow-fiber (HFM) bioreactor system that has been used to grow billions of functional T-cells in a short timeframe (8 to 9 days). We have now focused our protocol development on the ex vivo expansion of selected hTregs in the Quantum system using a soluble activator, composed of an anti-CD3/CD28/CD2 tetrameric mAb complex. Methods, Results & Conclusion hTreg CD4+CD25+CD127− cells from 3 unrelated healthy donors, previously isolated by the vendor via column-free Imag positive selection, were thawed and grown under static conditions and subsequently seeded into Quantum system HFM bioreactors and into T225 control flasks in an identical culture volume of 124 mL PRIME-XV® XSFM medium supplemented with 100 IU/mL of rhIL-2 and antibiotics for scale-up expansion over 9 days to compare the effects of automated perfusion with manual diffusion-based culture. Cell growth, viability, and phenotype of the hTregs were compared. The hTreg cell harvest from 3 parallel expansion runs produced an average of 4.0 × 108 (range 1.9 to 5.6 × 108) hTregs in the flask arm with an average viability of 71.3% versus 7.0 × 109 (range 3.6 to 13.0 × 109) hTregs in the Quantum arm with an average viability of 91.8%. This translates into an average 17.7-fold increase in hTreg cell yield for the Quantum system over that obtained in flasks, coupled with a higher cell viability in the Quantum system. In addition, these 2 culture processes gave rise to cells with an hTreg CD4+CD25+FoxP3+ phenotype of 76.5% for the flask arm versus 80.3% for the Quantum system arm. The results suggest that an automated perfusion HFM bioreactor can support the scale-up expansion of hTreg cells more efficiently than diffusion-based flask culture.
    • Randomized Comparison of Terumo® Coated Slender™ versus Terumo® Noncoated Traditional Sheath during Radial Angiography or Percutaneous Coronary Intervention

      Sindberg, Birthe; Aagren Nielsen, Christel Gry; Poulsen, Marianne Hestbjerg; Bøhme Rasmussen, Martin; Carstensen, Steen; Thim, Troels; Jakobsen, Lars; Sørensen, Jacob Thorsted; Haastrup, Benedicte; Søndergaard, Hanne Maare; et al. (2019-01-01)
      Background. The transradial approach is generally associated with few complications. However, periprocedural pain is still a common issue, potentially related to sheath insertion and/or arterial spasm, and may result in conversion to femoral access. Radial artery occlusion (RAO) following the procedure is also a potential risk. We evaluate whether the design of the sheath has any impact on these variables. Methods. A total of 1,000 patients scheduled for radial CAG or PCI were randomized (1:1) to the use of a Slender or a Standard sheath during the procedure. Randomization was stratified according to chosen sheath size (5, 6, 7 French) and gender. A radial band was used to obtain hemostasis after the procedure, employing a rapid deflation technique. A reverse Barbeau test was performed to evaluate radial artery patency after removal of the radial band, and level of pain was assessed using a numeric rating scale (NRS). Results. Use of the Slender sheath was associated with less pain during sheath insertion (median NRS 1 versus 2, p=0.02), whereas no difference was observed in pain during the procedure, radial procedural success rates, use of analgesics and sedatives during the procedure, and radial artery patency following the procedure. Rate of RAO was 1.5% with no difference between groups. Conclusion. The use of the hydrophilic coated Slender sheath during radial CAG or PCI was associated with less pain during sheath insertion, whereas no difference in other endpoints was observed. A rapid deflation technique was associated with RAO of only 1.5%.
    • Integrating phenotypic small-molecule profiling and human genetics: The next phase in drug discovery

      Johannessen, Cory M.; Clemons, Paul A.; Wagner, Bridget K. (2015-01-01)
    • Audiovisual Streaming Module Demonstration content

      Deroost, Ignace; Lowel, Art
      The content in this item is for Audiovisual Streaming module demonstration purposes.
    • Atmire test item 2019-07-22

      Luyten, Bram; Carberry, Josiah